Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Gly24-Ala155
Target:
IL-17A/CTLA-8
Biotinylated Human IL-17A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Human IL-17R alpha, hFc Tag captured on CM5 Chip via protein A can bind Biotinylated Human IL-17A, His-Avi Tag with an affinity constant of 2.18 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human IL-17A, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human IL-17A, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
The purity of Biotinylated Human IL-17A is greater than 95% as determined by SEC-HPLC.
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