Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Phe78-Val216
Target-Kategorie:
NKG2D/CD314
Human NKG2D on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Human MICB, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 95.15 nM as determined in SPR assay (Biacore T200).
Immobilized Human NKG2D, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ULBP-6, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA.
Immobilized Human NKG2D, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
Immobilized Human NKG2D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test).
The purity of Human NKG2D is greater than 90% as determined by SEC-HPLC.
Loaded Human ULBP-2, hFc Tag (Cat. ULB-HM202) on ProA-Biosensor can bind Human NKG2D, His Tag with an affinity constant of 1.16 µM as determined in BLI assay (Gator Prime).
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