Non-biotinylated Human NKG2D/CD314 Protein (NKG-HM42D)

Catalog Number: KAB-NKG-HM42D
Article Name: Non-biotinylated Human NKG2D/CD314 Protein (NKG-HM42D)
Biozol Catalog Number: KAB-NKG-HM42D
Supplier Catalog Number: NKG-HM42D
Alternative Catalog Number: KAB-NKG-HM42D-100UG, KAB-NKG-HM42D-500UG
Manufacturer: KactusBio
Category: Proteine/Peptide
Species Reactivity: Human
Alternative Names: CD314, D12S2489E, KLR, NKG2-D, NKG2D
Recombinant Human NKG2D/CD314 Protein is expressed from HEK293 with Avi tag and His tag at the N-Terminus.It contains Phe78-Val216.
Molecular Weight: The protein has a predicted MW of 19.0 kDa. Due to glycosylation, the protein migrates to 36-38 kDa based on Bis-Tris PAGE result.
Tag: N-Avi-His
UniProt: P26718
Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System: HEK293
Purity: > 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
Form: Lyophilized
Sequence: Phe78-Val216
Target: NKG2D/CD314
Human NKG2D on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Human MICB, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 95.15 nM as determined in SPR assay (Biacore T200).
Immobilized Human NKG2D, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ULBP-6, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA.
Immobilized Human NKG2D, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
Immobilized Human NKG2D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test).
The purity of Human NKG2D is greater than 90% as determined by SEC-HPLC.
Loaded Human ULBP-2, hFc Tag (Cat. ULB-HM202) on ProA-Biosensor can bind Human NKG2D, His Tag with an affinity constant of 1.16 µM as determined in BLI assay (Gator Prime).