GFAP Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-15-662
Artikelname: GFAP Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-15-662
Hersteller Artikelnummer: 15-662
Alternativnummer: PRS-15-662-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1).
Konjugation: Unconjugated
Alternative Synonym: Glial fibrillary acidic protein, GFAP, GFAP
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 50kDa
NCBI: 2670
UniProt: P14136
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using GFAP antibody (15-662) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using GFAP antibody (15-662) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat cerebellum using GFAP antibody (15-662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using GFAP antibody (15-662) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-251MG cells using GFAP antibody (15-662) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat brain using GFAP antibody (15-662) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse brain using GFAP antibody (15-662) at dilution of 1:100. Blue: DAPI for nuclear staining.