GFAP Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-15-662
- Images (7)
| Article Name: | GFAP Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-15-662 |
| Supplier Catalog Number: | 15-662 |
| Alternative Catalog Number: | PRS-15-662-100 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1). |
| Conjugation: | Unconjugated |
| Alternative Names: | Glial fibrillary acidic protein, GFAP, GFAP |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
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Western blot analysis of extracts of various cell lines, using GFAP antibody (15-662) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
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Immunohistochemistry of paraffin-embedded rat brain using GFAP antibody (15-662) at dilution of 1:200 (40x lens). |
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Immunohistochemistry of paraffin-embedded rat cerebellum using GFAP antibody (15-662) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse brain using GFAP antibody (15-662) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of U-251MG cells using GFAP antibody (15-662) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of rat brain using GFAP antibody (15-662) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of mouse brain using GFAP antibody (15-662) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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