PRKCE Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-18-509
Artikelname: PRKCE Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-18-509
Hersteller Artikelnummer: 18-509
Alternativnummer: PRS-18-509-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 488-737 of human PRKCE (NP_005391.1).
Konjugation: Unconjugated
Alternative Synonym: Protein kinase C epsilon type, nPKC-epsilon, PRKCE, PKCE
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 100kDa
NCBI: 5581
UniProt: Q02156
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using PRKCE antibody (18-509) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat spleen using PRKCE antibody (18-509) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using PRKCE antibody (18-509) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using PRKCE antibody (18-509) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PRKCE antibody (18-509) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human kidney using PRKCE antibody (18-509) at dilution of 1:100 (40x lens).