PRKCE Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-18-509
Article Name: PRKCE Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-18-509
Supplier Catalog Number: 18-509
Alternative Catalog Number: PRS-18-509-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 488-737 of human PRKCE (NP_005391.1).
Conjugation: Unconjugated
Alternative Names: Protein kinase C epsilon type, nPKC-epsilon, PRKCE, PKCE
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 100kDa
NCBI: 5581
UniProt: Q02156
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using PRKCE antibody (18-509) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat spleen using PRKCE antibody (18-509) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using PRKCE antibody (18-509) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using PRKCE antibody (18-509) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PRKCE antibody (18-509) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human kidney using PRKCE antibody (18-509) at dilution of 1:100 (40x lens).