SMAD9 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-23-001
Artikelname: SMAD9 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-23-001
Hersteller Artikelnummer: 23-001
Alternativnummer: PRS-23-001-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SMAD9 (NP_005896.1).
Konjugation: Unconjugated
Alternative Synonym: SMAD9, SMAD, mothers against DPP homolog 9 (Drosophila)], HGNC:6774, MADH6, MADH9, SMAD8A, SMAD8B, MAD (mothers against decapentaplegic, Drosophila) homolog 9, MAD, mothers against decapentaplegic homolog 9, mothers against decapentaplegic homolog 9 (Drosophila), Mothers against decapentaplegic, drosophila, homolog of, 9
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 45-52kDa
NCBI: 4093
UniProt: O15198
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using SMAD9 antibody (23-001) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human liver damage using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human brain cancer using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using SMAD9 antibody (23-001). Blue: DAPI for nuclear staining.