Western blot analysis of extracts of various cell lines, using SMAD9 antibody (23-001) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human liver damage using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human brain cancer using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using SMAD9 antibody (23-001). Blue: DAPI for nuclear staining.
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