| Chemokine receptors are specialized proteins that function at the surface of cells, and enable chemokines to trigger internal cellular processes. Chemokines and their receptors play a pivotal role in lymphocyte trafficking, recruiting and recirculation. Chemokines act through seven transmembrane G protein-coupled receptors. The chemokine system is increasingly recognized to play an important role in lymphocyte biology. Applications: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:500, binds to a ~40kD protein in Western blotting on mouse lung or human T cell line Jurkat and K562 cells Immunohistochemistry (paraffin): 1:150 ELISA: 1:50,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
