Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:
Unconjugated
Alternative Names:
CHMP7
Involved in several processes, including late endosome to vacuole transport, midbody abscission, and mitotic nuclear division. Located in cytosol, nuclear envelope, and nucleoplasm. Part of ESCRT III complex. Colocalizes with chromatin.
WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:
Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cell Biology Developmental Biology,Autophagy. Shipping: Ice Bag
Western blot analysis of various lysates using CHMP7 Rabbit pAb (A14360) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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