Cytokeratin 12 (KRT12) Rabbit pAb, Unconjugated, Polyclonal

Catalog Number: ABB-A14746
Article Name: Cytokeratin 12 (KRT12) Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number: ABB-A14746
Supplier Catalog Number: A14746
Alternative Catalog Number: ABB-A14746-100UL,ABB-A14746-20UL,ABB-A14746-1000UL,ABB-A14746-500UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: K12, MECD1, Cytokeratin 12 (KRT12)
KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.
Clonality: Polyclonal
Molecular Weight: 54kDa
NCBI: 3859
UniProt: Q99456
Purity: Affinity purification
Sequence: TRLLNDMRAQYETIAEQNRKDAEAWFIEKSGELRKEISTNTEQLQSSKSEVTDLRRAFQNLEIELQSQLAMKKSLEDSLAEAEGDYCAQLSQVQQLISNLEAQLLQVRADAERQNVDHQRLLNVKARLELEIETYRRLLDGEAQGDGLEESLFVTDSKSQAQSTDSSKDPTKTRKIKTVVQEMVNGEVVSSQVQEIEELM
Target: KRT12
Antibody Type: Primary Antibody
Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Mouse,Rat. ResearchArea: Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Intermediate Filaments,Neuroscience, Cell Type Marker. Shipping: Ice Bag
Western blot analysis of various lysates using Cytokeratin 12 (KRT12) Rabbit pAb (A14746) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.