IBSP Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A16220
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| Article Name: | IBSP Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A16220 |
| Supplier Catalog Number: | A16220 |
| Alternative Catalog Number: | ABB-A16220-20UL,ABB-A16220-100UL,ABB-A16220-1000UL,ABB-A16220-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | BSP, BNSP, SP-II, BSP-II, IBSP |
| The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. |
| Application Dilute: | WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Extracellular Matrix,Bone,Stem Cells,Mesenchymal Stem Cells. Shipping: Ice Bag |
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Western blot analysis of various lysates, using IBSP Rabbit pAb (A16220) at 1:2500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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