PDK1/PDPK1 Rabbit pAb, Unconjugated, Polyclonal

Catalog Number: ABB-A21810
Article Name: PDK1/PDPK1 Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number: ABB-A21810
Supplier Catalog Number: A21810
Alternative Catalog Number: ABB-A21810-20UL,ABB-A21810-100UL,ABB-A21810-500UL,ABB-A21810-1000UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: PDK1, PDPK2, PDPK2P, PRO0461, PDK1/PDPK1
Enables 3-phosphoinositide-dependent protein kinase activity, phospholipase activator activity, and phospholipase binding activity. Involved in several processes, including cell surface receptor signaling pathway, regulation of protein kinase activity, and regulation of signal transduction. Acts upstream of or within intracellular signal transduction. Located in cell projection, cytosol, and plasma membrane. Implicated in prostate cancer. Biomarker of lung non-small cell carcinoma.
Clonality: Polyclonal
Molecular Weight: 63kDa
NCBI: 5170
UniProt: O15530
Purity: Affinity purification
Sequence: GSNIEQYIHDLDSNSFELDLQFSEDEKRLLLEKQAGGNPWHQFVENNLILKMGPVDKRKGLFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKNFKTFFVHTPNRTYYLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAVQ
Target: PDPK1
Antibody Type: Primary Antibody
Application Dilute: WB,1:2000 - 1:6000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Signal Transduction,G protein signaling,Kinase,Serine threonine kinases,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Cytoskeleton,Microfilaments,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation,T Cell Receptor Signaling Pathway,NF-kB Signaling Pathway,Neuroscience. Shipping: Ice Bag
Western blot analysis of various lysates using PDK1/PDPK1 Rabbit pAb (A21810) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.