GPT2 Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A23670
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| Article Name: | GPT2 Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A23670 |
| Supplier Catalog Number: | A23670 |
| Alternative Catalog Number: | ABB-A23670-1000UL,ABB-A23670-20UL,ABB-A23670-100UL,ABB-A23670-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | ALT2, GPT 2, MRT49, NEDSPM, GPT2 |
| This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants. |
| Application Dilute: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human. ResearchArea: Cancer,Endocrine Metabolism,Amino acid metabolism. Shipping: Ice Bag |
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Western blot analysis of lysates from HepG2 cells, using GPT2 Rabbit pAb (A23670) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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