LRPPRC Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A3365
- Images (8)
| Article Name: | LRPPRC Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A3365 |
| Supplier Catalog Number: | A3365 |
| Alternative Catalog Number: | ABB-A3365-100UL,ABB-A3365-500UL,ABB-A3365-20UL,ABB-A3365-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | LSFC, GP130, LRP130, MC4DN5, CLONE-23970, LRPPRC |
| This gene encodes a leucine-rich protein that has multiple pentatricopeptide repeats (PPR). The precise role of this protein is unknown but studies suggest it may play a role in cytoskeletal organization, vesicular transport, or in transcriptional regulation of both nuclear and mitochondrial genes. The protein localizes primarily to mitochondria and is predicted to have an N-terminal mitochondrial targeting sequence. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome. |
| Clonality: | Polyclonal |
| Molecular Weight: | 158kDa |
| NCBI: | 10128 |
| UniProt: | P42704 |
| Purity: | Affinity purification |
| Sequence: | IACRLNQKKGAYDIFLNAKEQNIVFNAETYSNLIKLLMSEDYFTQAMEVKAFAETHIKGFTLNDAANSRLIITQVRRDYLKEAVTTLKTVLDQQQTPSRLAVTRVIQALAMKGDVENIEVVQKMLNGLEDSIGLSKMVFINNIALAQIKNNNIDAAIENIENMLTSENKVIEPQYFGLAYLFRKVIEEQLEPAVEKISIMAERLANQFAIYKPVTDFFLQLVDAGKVDDARALLQRCGAIAEQTPILLLFLLRNS |
| Target: | LRPPRC |
| Antibody Type: | Primary Antibody |
| Application Dilute: | WB,1:1000 - 1:5000|IHC-P,1:50 - 1:200|IF/ICC,1:100 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Endocrine Metabolism,Mitochondrial metabolism,Neuroscience,Neurodegenerative Diseases. Shipping: Ice Bag |
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Western blot analysis of various lysates, using LRPPRC Rabbit pAb (A3365) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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Immunohistochemistry analysis of paraffin-embedded Human breast cancer using LRPPRC Rabbit pAb (A3365) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Western blot analysis of various lysates, using LRPPRC Rabbit pAb (A3365) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 0.5s. |
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Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using LRPPRC Rabbit pAb (A3365) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Confocal immunofluorescence analysis of Hela cells using LRPPRC Rabbit pAb (A3365) at dilution of 1:400. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of A-549 cells using LRPPRC Rabbit pAb (A3365) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of HepG2 cells using LRPPRC Rabbit pAb (A3365) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of NIH/3T3 cells using LRPPRC Rabbit pAb (A3365) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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