Anti-SAMHD1 Antibody, Rabbit, Polyclonal

Article Name:	Anti-SAMHD1 Antibody, Rabbit, Polyclonal
Biozol Catalog Number:	BOB-A00592-1
Supplier Catalog Number:	A00592-1
Alternative Catalog Number:	BOB-A00592-1-0.1MG
Manufacturer:	Boster Bio
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC, WB
Species Reactivity:	Human, Mouse
Immunogen:	Anti-SAMHD1 antibody was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human SAMHD1.
Alternative Names:	SAM domain and HD domain 1, DCIP, CHBL2, HDDC1, MOP-5, SBBI88

Boster Bio Anti-SAMHD1 Antibody (Catalog A00592-1). Tested in ELISA, WB, IHC, IF applications. This antibody reacts with Human, Mouse.

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	72201 MW
UniProt:	Q9Y3Z3
Buffer:	SAMHD1 antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:

WB: 1 µg/mL, IHC: 5 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot, Immunohistochemistry, Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the rese

	Overexpression Validation in 293T Transfected CellsLoading: 15 &956,g of lysates per lane.Antibodies: SAMHD1 A00592-1 (0.1 &956,g/mL), (1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.293 cells were transfected with (1) wild-type SAMHD1 or (2) SAMHD1 (mutation T592A).
	KO Validation of SAMHD1 in xenograft mice (Kodigepalli et al., 2018) THP-1 control and SAMHD1 KO (THP-1/KO) cells were injected into NSG (non-obese diabetic/severe combinedimmune deficient-gamma) mice. Protein expression levels of SAMHD1 were examined by Western blot with anti-SAMHD1 antibodies (A00592-1) and SAMHD1 was not detected in THP-1/KO cells.
	Overexpression Validation of SAMHD1 in CD4+ T-cells from a healthy donor and transformed CD4+ T-cell lines (Kohnken et al., 2017) MT1, MT2, SLB-1, and C8166 were from leukemia patients and HH, HuT78, and HuT102 were from cutaneous T-cell lymphoma (CTCL) patients. SAMHD1 protein expression detected by anti-SAMHD1 antibodies (A00592-1) was significantly increased in normal CD4+ T-cells as compared to leukemia- and CTCL- derived CD4+ T-cell lines. This blot is representative from four independent experiments with four healthy donors.
	Overexpression of SAMHD1 in CD4+ T-cells from Healthy Donors and Sezary Syndrome (SS) patients(Kohnken et al., 2017)SAMHD1 protein expression detected by anti-SAMHD1 antibodies (A00592-1) was significantly reduced in CD+ T-cells from 15 SS patients as compared to those from 7 healthy donors.
	Regulated Expression Validation of SAMHD1 in CD4+ T-cells from a healthy donor (Kohnken et al., 2017)SAMHD1 protein expression detected by anti-SAMHD1 antibodies (A00592-1) was significantly decreased by about 40% relative to control cells at 48hr post-nucleofection with miR-181b.
	Regulated Expression Validation of SAMHD1 in MT2 CD4+ T-cells from leukemia patients(Kohnken et al., 2017)SAMHD1 protein expression detected by anti-SAMHD1 antibodies (A00592-1) was significantly increased by 5-fold at 48hr post-nucleofection with miR-181family inhibitor treatment.
	Immunofluorescence Validation of SAMHD1 in Human Daudi cellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Human Daudi Cells labeling SAMHD1 with A00592-1 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
	Immunohistochemistry Validation of SAMHD1 in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using anti- SAMHD1 antibody (A00592-1) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 &730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Western Blot Validation in Human Daudi Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: SAMHD1 A00592-1, 1 &956,g/mL, in (A: the absence and B: the presence of blocking peptide), (1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.