Phospho-Chk1 (S296) (14A3) Rabbit Monoclonal Antibody, Unconjugated
Catalog Number:
EKL-AMRE05875
Product Overview
Article Name:
Phospho-Chk1 (S296) (14A3) Rabbit Monoclonal Antibody, Unconjugated
Biozol Catalog Number:
EKL-AMRE05875
Supplier Catalog Number:
AMRe05875
Alternative Catalog Number:
EKL-AMRE05875-50UL, EKL-AMRE05875-100UL, EKL-AMRE05875-200UL
Manufacturer:
EnkiLife
Host:
Rabbit
Category:
Antikörper
Application:
WB
Species Reactivity:
Human
Conjugation:
Unconjugated
Alternative Names:
Checkpoint, S. pombe, homolog of, 1, CHEK1, CHK1, CHK1 checkpoint homolog (S. pombe), Serine/threonine-protein kinase Chk1,
Product Description
Chk1 kinase acts downstream of ATM/ATR kinase and plays an important role in DNA damage checkpoint control, embryonic development, and tumor suppression. Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. Activation occurs in response to blocked DNA replication and certain forms of genotoxic stress. Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA (PubMed:11535615, PubMed: 12446774, PubMed: 12399544, PubMed: 14559997, PubMed: 14988723, PubMed: 15311285, PubMed: 15665856, PubMed: 15650047, PubMed: 32357935). May also negatively regulate cell cycle progression during unperturbed cell cycles (PubMed: 11535615, PubMed: 12446774, PubMed: 12399544, PubMed: 14559997, PubMed: 14988723, PubMed: 15311285, PubMed: 15665856, PubMed: 15650047). This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome (PubMed: 11535615, PubMed: 12446774, PubMed: 12399544, PubMed: 14559997, PubMed: 14988723, PubMed: 15311285, PubMed: 15665856, PubMed: 15650047). Recognizes the substrate consensus sequence [R-X-X- S/T] (PubMed: 11535615, PubMed: 12446774, PubMed: 12399544, PubMed: 14559997, PubMed: 14988723, PubMed: 15311285, PubMed: 15665856, PubMed: 15650047). Binds to and phosphorylates CDC25A, CDC25B and CDC25C (PubMed: 9278511, PubMed: 12676583, PubMed:
Product Properties
Clonality:
Monoclonal
Molecular Weight:
54kDa
NCBI:
1111
UniProt:
O14757
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Purity:
Affinity purification
Form:
Liquid
Target:
CHEK1
Application Notes
Application Dilute:
WB 1:500-1:2000
