Applications: Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Conjugation Chemistry: N-hydroxysuccinimide (NHS) ester Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by ion exchange chromatography followed by tandum molecular sieve chromatography. IEP Assay shows a single precipitin arc against anti-biotin, anti-human IgM and anti-human serum. No reaction against anti human IgG F(c) or anti-pepsin.
Form:
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile ddH20.
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