Full-length purified human Siglec-1(d1-4)Fc protein. Cellular Localization: Isoform 1: cell membrane, Single-pass type-1 membrane protein, Isoform2: secreted. Species Sequence Homology: porcine, virus, and mouse.
The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumors. Applications: Suitable for use in Western Blot, RIA, Flow Cytometry, ELISA, Immunofluorescence/Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (frozen): 1:10-1:500 Immunohistochemistry (paraffin): 1:10-1:500 Immunohistochemistry: 1:10-1:500 Flow Cytometry: 1:10-1:1000 Immunofluorescence (IC): 1:10-1:500 ELISA: 1:100-1:2000 Western Blot: 1:100-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
